Microchipping of Animals

Literature Review

July 30, 2013

Animal Microchip Types and Frequencies

Implantable microchips are cylindrical devices that are implanted in the subcutaneous tissues using a hypodermic needle. These devices contain four components: a capacitor, antenna, connecting wire and a covering.1 The devices are battery-free and sealed in biocompatible glass covered by a sheath to prevent migration. Microchips are activated by a low-power radiofrequency signal emitted by scanners; ; electromagnetic induction generates electricity in the antenna and transmits the information stored in the microchip.1 . When activated by the scanner, the microchip transmits a unique, preprogrammed identification number.2,3 Some microchips used in animal research also collect and transmit body temperature data.4
 
Microchips are produced by various manufacturers within the U.S. and other countries. The metal composition of the microchip varies among manufacturers, and may consist of ferrite, titanium, or other metals.1 The microchip surface materials are usually bioglass (biocompatible glass) or bioglass coated with different polymers.5 Radio Frequency Identification Device (RFID) microchips are implanted in animals.

Differences in animal microchip frequency in the US have led to controversy and several civil lawsuits.3,6,7 There is no agreed-upon "American standard" for microchip frequencies.7 The American National Standards Institute (ANSI) voted in favor of the current International Standards Organization (ISO) Standards both at the time of initial adoption in 1996 and at the time of their mandatory 5-year review in 2001 and 2006. In its August 2006 report to USDA, the Equine Species Working Group urged use of the ISO standard for identification of equine species, as well as the development of ISO-compliant scanners that are also able to read, or at least detect, 125-kHz microchips.8

The ISO standards 11784/11785 were implemented in 1996.9 They are accepted by Canada, Europe, Asia, and Australia, and have been endorsed for use in the United States by the American National Standards Institute (ANSI). ISO standard 11784 defines the structure of the microchip information content, and standard 11785 determines the protocol for scanner-microchip communication.10 The standards include the assignment of a 15-digit numeric identification code to each microchip: 3 digits of the identification number designate either the 3-digit country code of the country in which the animal was implanted (The Country code is used only if there is a central, national database that takes on the responsibility of administering number allocation and ensuring no number duplication) or the 3-digit manufacturers code (as assigned by the International Committee on Animal Recording [ICAR]) if there is no central, national database, to ensure number uniqueness; 1 digit denotes the animal's category (optional); and the remaining 8- or 9-digits denotes a unique animal identification number. In 2005, a dog was reunited with its owner in Toronto, Canada after its ISO microchip number was traced to its original registration in Portugal.10 Non-ISO standard (125- or 128-kHz) microchips contain 9 or 10 digits and do not include a country code. Manufacturer codes are assigned by ICAR following an application and review process that includes signing a "code of conduct." A list of Manufacturer Codes can be found at on the ICAR site.

Commonly available microchips implanted in pets in the U.S. include the following:

125-kHz microchips:
24PetWatch® (unencrypted)
FriendChip®, Avid (encrypted)
HomeAgain®/Digital Angel (unencrypted)

128-kHz microchips:
AKC Companion Animal Recovery® (AKC CAR®)

134.2-kHz microchips:
Bayer ResQ®
HomeAgain®/Digital Angel

Animal Microchipping in the United  States and Other Countries

The ISO approves 134.2-kHz as the standard frequency for animal microchips, but 125-kHz, non-ISO microchips remain the predominant microchip frequency used within the US at this time. In recent years, additional 128-kHz and ISO-compliant 134.2-kHz microchips have been introduced.11 The American Veterinary Medical Association (AVMA), American Animal Hospital Association (AAHA), World Small Animal Veterinary Medical Association (WSAVA), and American Society for the Prevention of Cruelty to Animals (ASPCA) endorse the use of electronic identification in animals and support the implementation of ISO standard 11784/11785 in the U.S. The AVMA's policy on "The Objectives and Key Elements Needed for Effective Electronic Identification of Companion Animals, Birds, and Equids" states "The AVMA endorses the use of electronic identification in animals and supports the standardization in materials, procedures, equipment, and registries. Veterinarians are thereby encouraged to recommend the use of electronic identification of animals to their clients." The implementation of a global system of animal identification has the potential to eliminate geographical boundaries that might interfere with animal recovery.
 
At this time, microchipping of pets and other animals is voluntary in the U.S. except for some legislation mandating microchipping as a means of identifying animals who have been identified as being dangerous. In 1994, the Louisiana Department of Agriculture and Forestry (LDAF) issued a regulation requiring permanent identification (in the form of a brand, lip tattoo or electronic identification) of all horses tested for equine infectious anemia (EIA).12 According to the LDAF and the state veterinarian, this requirement was "a significant help" in determining the owners of horses displaced during Hurricane Katrina in fall 2005.12,13
 
Language was inserted into the 2005 Agriculture Appropriations Bill supporting the use of microchip implantation in pets for identification purposes. As a result, Congress directed the US Department of Agriculture Plant Health and Inspection Service (USDA-APHIS) to develop regulations for microchip identification of pets. In July 2007, the USDA-APHIS released a report to Congress regarding microchipping of pets in the United States. Because the Animal Welfare Act does not authorize the USDA-APHIS to regulate private pet ownership, the organization concluded that it cannot mandate a national standard for pet microchips or scanners.14,15

The National Animal Identification System (NAIS) was implemented to protect the health and economic viability of US livestock and poultry.16 The program as originally proposed involved the assignment of a premises identification number (PIN) to each participating premises, and an animal identification number (AIN) to each animal; animals of the same species that were to move through the production chain as a group could be assigned a group/lot identification number (GIN). The plan originally called for individual animals to be marked with NAIS-compliant RFID tags or implantable microchips; however, based on industry backlash, the plan was amended to remove the microchip requirement. In 2007, the USDA-APHIS announced its support of ISO standards but remains neutrally positioned on specific identification technology.14

A survey conducted by the WSAVA in November 2002 revealed that Western Europe, Australia, and New Zealand had successfully adopted and implemented ISO standards; Eastern Europe, the Middle East, and Asia demonstrated market dominance of ISO-standard microchips; Canada and South America were developing an increased market for ISO-standard microchips; and the U.S. and Africa demonstrated the least development and compliance with the standards.17

In December 2004, Canada's National Companion Animal Coalition (NCAC) adopted ISO standards 11784 and 11785 for microchip identification of companion animals. As of August 1, 2005, only ISO standard microchips are recognized by the NCAC as suitable for the electronic identification of companion animals in Canada. ISO standards 11784/11785 are also accepted by Europe, Asia and Australia.6, 18

In 2006, the European Union (EU) proposed a regulation requiring foals to be microchipped before applying for an equine passport.19 Currently, an equine passport is required for all horses in EU countries, regardless of their use; this requirement was primarily implemented to prevent human consumption of horses that had received unapproved medications. Present EU regulations do not mandate microchip implantation of horses.

In September 2009, Dogs Trust campaigned for compulsory microchipping of all dogs in the U.K. in response to high reported numbers of stray dogs. The proposed legislation is an amendment to the Statutory Instrument 2006 No. 798, The Dog Control Orders (Procedures) Regulations 2006, The campaign has attained bipartisan support.20

Microchip Implantation

The use of standard microchip implantation sites reduces the risk of failure to detect an implanted microchip.21 As a general rule, the microchip should be implanted in dogs and cats so that its long axis is parallel to the animal's longitudinal axis.11
 
For a list of veterinary-recommended implantation sites in numerous animal species, go to the World Small Animal Veterinary Association (WSAVA) Microchip Web site.

Implantation of a microchip results in transient inflammation at the site of implantation, followed by long-term formation of a fibrous capsule around the microchip.5,22 In horses, a local inflammatory response and increased sensitivity to pressure at the insertion site resolved within 3 days and a systemic inflammatory response was not invoked. 22

The improper implantation of microchips can result in potentially life-threatening sequelae. The inappropriate, forceful implantation of a microchip by its owner resulted in microchip placement in the spinal canal of a 2-year old cat, causing tetraparesis (weakness of all four limbs) and tachypnea (increased breathing rate). The microchip was surgically removed and the cat recovered, but with residual, mild neurologic deficits.23 In two separate case reports, inappropriate microchip implantation into the spinal canal of small-breed puppies resulted in acute-onset tetraparesis that gradually resolved following surgical removal of the microchip.24,25 According to one report, an alpaca died acutely due to the inappropriate implantation of a microchip into its spinal canal.26 Delayed-onset forelimb lameness and ambulatory tetraparesis were reported in a 3-year-old Yorkshire Terrier due to improper microchip implantation into the vertebral canal; the authors stated that the British Small Animal Veterinary Association (BSAVA) guidelines for microchip placement were amended to recommend inserting the needle to only half its length when implanting microchips in puppies or kittens.27

In the U.K., microchip insertion is not considered a veterinary practice.25,28,29 The AVMA's "The Objectives and Key Elements Needed for Effective Electronic Identification of Companion Animals, Birds, and Equids" policy states that "implantation of microchips is a veterinary procedure that should be performed by a licensed veterinarian or under supervision of a licensed veterinarian."

Benefits of Microchipping Animals

Permanent, unalterable identification of animals can be a challenge. Although an accepted form of identification, the tattooing procedure can produce discomfort and tattoos can fade with time and can sometimes be altered. Identification tags are effective means of identification only if they are in place on the animal when it becomes lost. Ear tags are effective and visible means of identification, but can be removed intentionally or by trauma. Hot branding provides permanent identification of livestock, but its use in horses elicits a marked pain response followed by local inflammation and increased skin sensitivity for one week.30 Microchip implantation provides a reliable, and often less painful, method of permanent, unalterable animal identification.

 

The primary benefit of microchip implantation in pets is the increased chance of reunification of lost or stolen animals with their owners.31,32 Microchip implantation in horses and other livestock allows increased recovery of stolen animals and traceability in the event of a disease outbreak.

Lord et al33 determined that the owners of almost three-fourths of lost, microchipped cats and dogs were located due to the presence of the microchip. Owners were more likely to be found for dogs, purebred animals and spayed/neutered animals.33-37 The rate of return to owners was also higher for microchipped stray animals.33-37 When compared to a study34 that determined an owner's efforts were successful in recovering their dog only 13% of the time, the detection of a microchip by an animal shelter yielded a 74.1% rate of return to owners.

Dogs have been reunited with their owners years after they were lost, based on the detection of an implanted microchip.38-41 Dogs that were found 600 and 1000 miles from their homes were reunited with their owners due to the presence of microchips.42,43 In 2007, actress Vanessa Williams was reunited with her dog due to the presence of an implanted microchip.44

During the response effort to the floods of Hurricane Floyd in 1999, all animals received by the field hospital at the North Carolina State University College of Veterinary Medicine were implanted with microchips to facilitate identification and tracking.45 One of the recommendations put forth as a result of the 2006 National Animal Disaster Summit is that all animals rescued during a disaster should be implanted with microchips (if not already implanted) to facilitate identification, tracking and reunification with owners.46 In the aftermath of Hurricanes Katrina and Rita (2005), implanted microchips facilitated the identification of rescued animals.47

The Canadian livestock identification process has been driven by the livestock industry, and the mandatory identification system for cattle facilitated the epidemiological investigation of bovine spongiform encephalopathy (BSE).48

The use of thermal sensing microchips in horses has been promoted as a potential means of monitoring individual animals' body temperatures while decreasing the risk of disease transmission by rectal thermometers shared between multiple animals.49 Robinson et al49 determined that the thermal microchips produced more variation in temperature readings than rectal thermometers and the accuracy of the temperatures obtained by the microchip were strongly influenced by the ambient temperature. The thermal microchip over- or underestimated the animal's body temperature and failed to detect more than 50% of fevers in cooler ambient temperatures and approximately 15% of fevers during warmer ambient temperatures. Overall, the thermal sensor detected only 87% of fevers.

Microchip Scanners: Challenges and Efficacy

Detection of the presence of a microchip is the crucial third step of the reunification process (with the first step being implantation of the microchip and the second step being registration of the microchip). A survey-based demographic assessment of animal care and control agencies from 1996 to 2004 revealed an increase in the number of agencies that scanned for microchips upon the animal's arrival in 2004 compared to 1996.35 In addition, the number of agencies that implanted animals upon adoption increased from 4% to 8% over the same time period.35
 
Market competition and intentional incompatibility between manufacturers' microchips and scanners have led to controversy and lawsuits. According to the USDA-APHIS in 2007, approximately 3 to 5 % of pets within the U.S. are microchipped.50 Of these, 98% are implanted with 125-kHz microchips. Although there appears to be no independent survey data to verify the claim, the USDA-APHIS letter to Senator Byrd in 200750 commented that 80% of microchip scanners in the U.S. read only 125-kHz microchips. As new companies enter the U.S. microchip marketplace, this percentage will change. Two manufacturers currently hold the patent rights on the 125-kHz technology, including the scanners.51 One manufacturer sells encrypted 125-kHz microchips that can only be read by scanners authorized by that manufacturer; unauthorized scanners might detect the presence of a microchip, but cannot read or display the identification number. Scanners manufactured to detect only 125-kHz microchips cannot read or determine the presence of ISO microchips.52 Another brand of microchip with 128-kHz frequency has been marketed within the U.S.; this microchip is not detected by some 125-kHz scanners.

According to the Coalition for Reuniting Pets and Families (2005),53 less than 25% of lost pets in the U.S. are reunited with their owners. In contrast, 47% of lost dogs are reunited with their owners in the United Kingdom, where ISO standard chips are available and a more efficient database is utilized.53

Animals traveling to member countries of the European Union (EU) or other countries that have adopted the ISO 11784/11785 standards must be implanted with microchips that meet those standards, or the pet owner may need to carry with them a scanner capable of reading the implanted non-ISO microchip.

The introduction of an ISO standard microchip in the United States in January 2004 underscored the need for scanners capable of detecting ISO and non-ISO microchips (also called 'universal scanners' or 'forward- and backward-reading scanners'). That same year, the controversy surrounding microchip frequency standards gained national attention when a lost pet dog implanted with a 134.2-kHz (ISO) microchip was euthanatized because the animal shelter's 125-kHz scanner did not detect the microchip.51 Ongoing efforts have been directed at the production of scanners capable of detecting and reading both ISO and non-ISO microchips. In 2007, two companies in the U.S. were engaged in an initial distribution of 50,000 universal scanners.54,55

Scanner abilities as reported by manufacturers:

Scanner Name

125-kHz (E)

125-kHz (UE)

128-kHz

134.2-kHz

ResQ®
(iMax Black Label)

Read Read Read Read

HomeAgain®
(Universal WorldScan; Digital Angel)

Read Read Read Read

AKC CAR®
(Multi-System Pocket Scanner; Trovan)

Read Read Read Detect Only

Avid
(MiniTracker 1 Universal Multi-Scan)

Read Read - -

E= encrypted; UE=uncencrypted

Lord et al11 tested 3 universal scanners and 1 125-kHz scanner in vitro and determined that even under controlled conditions, none of the scanners were 100% effective in detecting all microchips in all orientations. The universal scanners were able to detect ISO-compliant 134.2-kHz microchips more readily than 125- or 128-kHz microchips. Two of the scanners (HomeAgain® and Avid) were not affected by the orientation of the scanner relative to the microchip's long axis, whereas scanning orientation negatively affected the sensitivity of the AKC CAR scanner for 125-kHz microchips and, to a lesser extent, the Bayer scanner for one brand of 125-kHz and one brand of 128-kHz microchips. The following table summarizes the findings. For more information, please consult the full manuscript.

Sensitivity of commercial scanners to microchips of various frequencies

Scanner and Orientation

125-kHz (E)

125-kHz (UE)
(two brands)

128 -kHz

132.4-kHz
(two brands)

ResQ®, PL

100% 98.5%, 98.5% 99.6% 99.9%, 100%

ResQ®, PD

99.3% 100%, 86.5% 98.3% 99.9%, 100%

HomeAgain®, PL

99% 100%, 100% 98.1% 100%, 99.9%

HomeAgain®, PD

100% 100%, 100% 96.0% 100%, 100%

Avid, PL

100% 99.7%, 99.7% n/a n/a

Avid, PD

99.9% 99.7%, 99.7% n/a n/a

AKC CAR®, PL

53.5% 83.2%, 71.7% 99.9% 99.9%, 99.9%
(detect only; did not read)

AKC CAR®, PD

81.8% 97.6%, 97.1% 99.7% 100%, 99.9%
(detect only; did not read)

E=encrypted; UE=unencrypted; PL=parallel to long axis of microchip; PD=perpendicular to long axis of microchip

Lord et al's in vitro findings11 correlated well with in vivo testing of the same scanners and microchips.56 Scanners capable of reading 128-kHz or 134.2-kHz microchips had sensitivities =94.8%. Sensitivity detecting 125-kHz microchips was lower, but still was equal to or greater than 88.2%.

Scanner

125-kHz (E)

125-kHz (UE)
(two brands)

128-kHz

134.2-kHz
(two brands)

HomeAgain®

95.9% 95.6%, 93.6% 95.2% 94.8%, 98.4%

ResQ®

92.1% 97.0%, 88.2% 97.0% 98.4%, 98.4%

AKC CAR®

75.0% 66.4%, 66.6% 98.9% 95.9%, 96.8%
(detect only; did not read)

Avid

98.2% 97.3%, 99.6% n/a n/a

Lord et al56 also observed that every 2.3-kg increase in body weight was associated with a 5% increase in the odds that a 125-kHz microchip would be missed and an 8% increase in the odds that a 128- or 134.2-kHz microchip would be missed.

Microchipped animals should be scanned during their regular preventive care/wellness exams to confirm that the microchip transponder remains functional. In addition, the client should be reminded at that time to check their registration information to make sure it is up-to-date.58

Scanning technique

Scanner performance and microchip detection can be affected by the following:11

  • Scanning orientation (perpendicular or parallel to the long axis of the microchip);
  • Distance between the microchip and scanner;
  • Scanner antenna tuning (better performance with narrower frequency tuning);
  • Threshold power needed to activate microchip (this depends on the power output of the scanner and the energy needed to activate the microchip);
  • Variations in microchip implantation technique;
  • Animal compliance during implantation and/or scanning; and
  • Microchip migration.

Based on in vitro and in vivo studies, Lord et al11,33,56,57 determined several criteria to maximize the efficacy of microchip scanning:

  • Obtain a universal scanner that can read microchips of all frequencies with high sensitivities.
  • Provide training on proper and consistent scanning technique for all personnel.
  • Avoid interference by scanning away from computers, metal tables and fluorescent lighting. Remove metal collars before scanning.
  • For the iMax Black Label, Home Again Pocket Reader and AVID Mini Tracker scanners, begin with the scanner parallel to the animal. The Trovan Pocket Scanner should be held perpendicular to the animal and the scan should be started with the scanner parallel to the animal’s spine.
  • Pass the scanner over the entire animal multiple times and in different orientations, with an “S”-shaped pattern from side to side. If a microchip is not detected, rotate the scanner 90 degrees and repeat the scan in an “S”-shaped pattern in a longitudinal direction. Be sure to scan the entire surface of the animal’s body. Hold the scanner in contact with the animal during the scanning process.
  • Begin scanning at the standard implantation site (midway between the shoulder blades) and concentrate on this area. If a microchip is not detected, scan the entire animal.
  • Do not scan any faster than ½ foot per second.
  • Scan the animal more than once. In shelters, animals should be scanned at intake, at medical processing, before euthanasia and before adoption.
  • Veterinary clinics should scan microchipped animals during every wellness examination to ensure continued microchip function.
  • Develop a regular battery change schedule for the scanner to avoid detection failure. Use high-quality batteries in scanners.

Microchip Registration Databases

Successful tracking and reunification of lost animals with their owners also rely on accurate information within a registration database. The microchip only contains a registration number; without accurate registration associated with the microchip number, a lost, microchipped animal that is scanned might not be returned to its owner. The U.S. is the only country in which microchip implantation and microchip registration are often separate processes.33 The lack of a centralized database in the U.S. has led to concerns of poor efficiency in reuniting lost pets with their owners.58 Lord et al37 determined that only 58.1% of microchipped animals were registered when animal shelters attempted to locate the animal's owner. In addition, the major reason animal shelters were unable to locate animal owners was incorrect owner information.
 
The American Animal Hospital Association (AAHA) created the AAHA Universal Pet Microchip Lookup Tool. The Tool, available at www.petmicrochiplookup.org, allows users to enter a microchip code and directs them to participating microchip registries associated with that microchip's number and the microchip's manufacturer.59  The site does not provide registration information and only provides information linking to the six microchip databases that operate in the U.S. Although a central database and/or search engine will facilitate identification of the microchip manufacturer, it is still incumbent on animal owners to register the microchip and keep the information updated.

A number of online microchip registries have launched since 2009, but many of these registries are stand-alone products that are not linked to manufacturer registries. Fortunately, some of these registries have been linked to the AAHA Universal Pet Microchip Lookup Tool; however, pet owners who choose to register with these independent registries must also register the microchip with the manufacturer’s registry. All registries should be updated as needed.

In the U.K., the BSAVA encourages veterinarians to perform the registration to ensure completion of the process.

A survey conducted by the WSAVA in November 2002 revealed that modifications of the ISO standard identification codes had been made by a number of countries, some of which were in violation of ISO/ICAR protocol. WSAVA defined a national database as one satisfying the following criteria: industry and species neutral; national authority to administer the database; and responsible for allocating microchip identification numbers and verifying uniqueness of the numbers. Based on these criteria, a true, national database did not exist in any of the 32 countries surveyed except for France.17

Lord recommends that the process of implanting a microchip never be separated from the registration process, and that veterinarians and shelters support the registration process to maximize efficacy. This can be accomplished by collecting information and completing the initial registration on behalf of the pet owner. 57

In 2013, the AVMA and AAHA joined together to designate August 15 of each year as “Check the Chip Day,” to serve as a reminder to all pet owners to check and update their pet’s microchip registration or to microchip and register their pets if they are not already microchipped.

Adverse Microchip Reactions and Biocompatibility

Transponder failures occur, but are rare. Transponder migration, although infrequent, is the most common complication associated with microchip implantation,28,29,61-63 with the elbow and shoulder the most common locations for errant transponders in small animals.28,29,62,63 Microchips implanted in the shoulder regions of dogs appear more likely to migrate.64 Retrospective evaluation of 33 horses, donkeys, and mules revealed that microchips implanted in the recommended site (nuchal ligament) according to manufacturer's recommendations did not migrate and were readily detected with scanners.2 Lord et al (2010) reported that transponder migration occurred in only 3 (0.6%) of 478 cats and the transponder failed in 1 cat (ie, the microchip failed to transmit the identification number when scanned).57

 

Microchip implantation should not be considered a substitute for proper external identification of animals.56 Microchipped animals should also wear collars with proper identification.34,36,37 The value of implanted microchips may be higher, and the chance of recovery improved, for cats that do not wear collars or easily escape collars.34,36,37 Tattoos, license tags, rabies tags and personal visual identification are all components of a comprehensive pet identification program.56

The BSAVA instituted a microchip adverse reaction reporting system in 1996. The following table summarizes the reported types and incidences of microchip-associated adverse reactions in the United Kingdom.61 The unified microchip registration database in the U.K, petlog, reports more than 3.7 million registered, microchipped pets.65 The following chart summarizes the adverse reactions reported to BSAVA:61

Reaction

2009

2008

2007

2006

2005

2004

2003

2002

2001

2000

1999

1998

1997

1996

Failed

    5 1 2 7 6 1 7 4 1 1 1  

Hair Loss

          1                

Infection

1   1 5 1 2 1 1 1 2 1 2 1 1

Lost

    3 3 7 6 6 2 3 34 7 1    

Migration

3 16 16 12 27 33 12 7 9 33 28 24 9  

Swelling

  1 3 8   1 3 2 1 1 2 1    

Tumor

        1   1              

Unknown

  1 1 3           1 2      

Total

4

18

29

32

38

50

29

13

21

75

41

29

11

1

 

Implantation of transponders into the subcutaneous tissues behind the ears of 4-week old piglets produced an initial inflammatory reaction that subsided by 21 days postimplantation. A fibrous capsule formed around the microchip and remained the same thickness from 21 days until the completion of the study at 6 months postimplantation.66 Microchip implantation in the subcutaneous tissues behind the ears of 55-week old piglets for intervals of 3 to 150 days was well accepted and produced a cellular tissue reaction that decreased with time.67

A study of 343 1- to 3-month old fattening calves implanted with a total of 686 injectable microchips in the ear, armpit, or upper lip revealed minimal tissue reaction surrounding the implants at slaughter 8-9 months after implantation.68

 The presence of a microchip can produce a susceptibility artifact on magnetic resonance imaging (MRI), which may lead to difficulty in interpreting the MRI in the region in which the microchip is implanted.1 However, the microchip is not damaged, altered, or migrated by the magnetic forces applied during MRI procedures, and the tissues surrounding the microchip are not adversely affected.1,69

Animal Microchips and Cancer

Microchip transponders are commonly used to facilitate identification of individual animals in research. Inbred mice strains are commonly used in research, and tumor susceptibility varies with strain.4,70-73
 
In species or strains prone to developing tumors, an implanted microchip may induce a foreign-body reaction and tumor formation, similar to vaccine-associated sarcomas in cats.71,74-76 The most commonly reported foreign body-induced tumors in mice and rats are fibrosarcomas, spindle-cell sarcomas, and anaplastic sarcomas.70 Implantation of glass coverslips, plastic coverslips, or plastic coverslips with roughened surfaces underneath the skin of inbred mice (CBA/H; CBA/H-T6; C57BL/10 ScSn; and AKR/J strains) resulted in the formation of foreign body-induced sarcomas.74 The tumor types identified were fibrosarcomas, spindle-cell sarcomas, round cell sarcomas, anaplastic sarcomas, one hemangiosarcoma, and one round-cell sarcoma with bone formation.74 Similarly, the implantation of nonreactive materials such as platinum, stainless steel, quartz, gold, and chemically pure polymers induced tumors in mice or rats.70

The risk of foreign body-induced tumors is affected by duration, species (and strain), and size.4,70 Mice and rats are more susceptible than other species to developing foreign body-induced tumors; therefore, extrapolation of increased incidences of foreign body-induced tumors in mice to increased risk in other species, including humans, is inappropriate.69 Any foreign substance inserted into the body for long periods of time is capable of inducing neoplasia.4,70,76 The surface texture of the foreign body affects the risk of reaction and neoplasia formation.4,70,77,78 The surface area of the foreign body is also a factor; larger surface areas are associated with increased risk of neoplasia.4,70 Although microchip size may vary within a narrow range, the majority of microchips used in research and companion animals in the United States are 2 mm in diameter and 12 mm in length. Therefore, the same size microchip in a mouse presents a markedly larger surface area compared to the animal's body size than it does in a larger species such as a dog or cat; this may partially explain the species differences in the reported incidence of tumors associated with microchip implantation.

A number of studies have reported microchip-associated tumor formation in laboratory animals. However, the majority of these studies were not conducted to determine the frequency of tumor formation associated with microchip implantation alone; the tumors were observed in animals used for ongoing carcinogen and oncogenicity research, and the possible impact of the carcinogenic substances on the reported incidence of tumor formation should be considered when interpreting the studies. In addition, the microchips implanted in these animals were not the type same microchips that are implanted into pets.

Ball et al78 reported minimal tissue reaction and an absence of tumor formation surrounding the microchip implantation sites of 40 Sprague-Dawley rats one year after implantation.

Elcock et al4 reported a 0.7% incidence of microchip-associated tumors during two 24-month, separate chronic toxicity/oncogenicity studies using a total of 1200 Fischer 344 rats. All tumors occurred during the second year of the studies, and were mesenchymal in origin. Eight rats developed visible tumors associated with the implanted microchips, and 5 of the 8 were euthanatized due to the tumors. None of the tumors occurred in untreated, control rats in either study.

Johnson75 reported an overall incidence of less than 1% of subcutaneous sarcomas identified in mice used for oncogenicity studies. The tumors occurred at one year post-implantation or later, and were typical of foreign body-induced sarcomas.

Following use of implanted microchips for identification of laboratory animals for more than a decade without tumor formation, one group of researchers observed an increased incidence of sarcomas in heterozygous p53+/- mice used for a carcinogenicity study.73 Eighteen of 177 mice (10%) developed sarcomas at the site of microchip implantation. However, the authors cited a previous reference supporting increased susceptibility of this strain of mouse to development of sarcomas at sites of foreign body implantation. In addition, the authors stated that use of the same transponder in more than 2000 mice of a different strain (Tg.AC) over a 4-year period did not result in the formation of tumors at the implantation sites.

Thirty-six (0.8%) of 4279 implanted mice (4279 CBA/J inbred strain) developed various soft tissue tumors at microchip implantation sites during research to investigate the influence of parental preconceptual exposure to radiation or chemical carcinogens.71 The majority of neoplasms were fibrosarcomas, but malignant fibrous histiocytomas, hemangiopericytoma-like neoplasms, and malignant schwannoma-like neoplasms were also identified. Evidence of metastasis was not observed in any of the affected animals. The frequency of tumor formation was significantly higher in females than males, but the general health of the mice was considered normal and no clinical signs were observed except nodules at the site of implantation.

The same microchip brand implanted into a different strain (B6C3F) did not induce tumor formation during a 24-month duration study.80 Mice used in this study (140 total) were not used for concurrent carcinogenicity or oncogenicity studies. The survival rate for the study did not differ from previous noninvasive, nutritional studies. Histologic examination of tissues obtained at 3-, 15-, and 24-month necropsies revealed a fibrous capsule of varying thickness encasing the implants; no neoplastic changes were observed.

Palmer et al72 reported fibrosarcomas associated with implanted transponders in 16 (2%) of 800 B6C3F1/CrlBR VAF/Plus mice. Four additional animals also developed fibrosarcomas, but the tumors in these mice were not associated with the implantation site. According to Rao and Edmondson,80 the prevalence of subcutaneous sarcomas in B6C3F1 mice was reported as high as 12%. Palmer et al reported no implant-associated tumors were identified in a different strain of mice (Crl:CD-1), supporting strain-related differences in susceptibility to tumor formation.

Le Calvez et al81 reported 4.1% of 1260 microchip-implanted mice used for 3 carcinogenicity studies developed tumors associated with the implanted transponders. The rate of tumor formation varied from one study to another, possibly reflecting an impact of the carcinogen studied. Fifty of the 52 mice that developed tumors were affected by mesenchymal tumors, and histopathologic examination of the remaining 2 tumors revealed mammary gland adenocarcinoma. The mice in these studies were B6C3F1 genetically modified mice; the authors reported a 2% baseline occurrence of these tumors within the strain population.

A microchip-associated leiomyosarcoma was reported in an Egyptian fruit bat housed in a bat conservancy.79 The microchip was embedded in the tumor, and both were excised. The bat died during the 5th postoperative week; necropsy revealed metastatic lesions in the peritoneal cavity. The authors stated that three bats of a population of 421 microchip-implanted bats over a 14-year period developed microchip-associated complications; two developed abscesses due to poor technique, and the third developed neoplasia as described in the report. The role of previously diagnosed hemochromatosis in the development of the tumor was unknown.

Soft tissue sarcomas associated with microchips were identified in a 4-year old, female degu and a 6-year old female feathertail glider.82 A fibrosarcoma was diagnosed in the degu 9 months following the implantation of the microchip. The feathertail glider developed osteosarcoma surrounding the microchip 5 years after implantation.

A long-term, controlled study of the effects of microchip implantation in dogs was reported, and short-term studies have demonstrated good biocompatibility and minimal tissue reaction around implanted microchips. Murasugi et al83 evaluated the effects of microchip implantation and readability in 9 dogs up to six years after implantation. The animals were anesthetized and the implanted microchips excised at 3 days, 3 months, 12 months, or 36 months after implantation. A foreign body reaction was histologically observed following implantation, but subsided within 3 months and was followed by the formation of a fibrous capsule. The fibrous capsule was unchanged from 12 months to 72 months after implantation.

Excision and examination of microchip implantation sites from cats, obtained 21 days after implantation, showed no inflammation associated with the microchip.84 Histopathologic evaluation of the tissues surrounding the implantation sites of a total of 90 RFID microchips for 16 weeks in 15 Beagle dogs revealed the formation of a noninflammatory, fibrous tissue capsule around 87 of the 90 implanted devices.64

The first report of a microchip-associated tumor in a pet was published in 2004.77 The dog, an 11-year old male, mixed-breed dog, developed a liposarcoma at the site of a microchip implanted craniodorsally to the top of the left shoulder blade (a common site for microchip implantation as well as vaccine administration). The owner observed a small nodule at the site of implantation approximately 18 months after implantation. The nodule increased in size, and was removed 3 years after initial implantation. The microchip was embedded in the tumor. Complete surgical excision was performed, and no signs of recurrence were observed 3 months after surgery.

The findings reported by Vascellari et al in 200477 report were similar to those reported by McCarthy et al in 1996,85 when an 11-year old spayed female, mixed-breed dog developed a liposarcoma associated with a glass foreign body. The authors determined the glass had become implanted when the dog had fallen from a pickup truck onto a gravel roadway 10 years prior to discovery of the mass. The foreign body and associated tumor were excised, and no tumor recurrence was observed one year after excision.84

The second report of a microchip-associated tumor was published by the Vascellari et al76 in 2006. A 9-year old, male French Bulldog developed a subcutaneous mass near the site of microchip implantation and vaccine administration approximately 8 months prior to tumor development. Histopathologic evaluation revealed infiltrative fibrosarcoma, similar to postinjection sarcomas observed in cats. In contrast to the prior case, the microchip in this case was attached to, but not embedded in, the tumor; based on the findings, the authors were unable to determine if the tumor was induced by the implanted microchip, rabies vaccines, or a combination of factors. Microchips are often implanted in sites commonly used for subcutaneous injection of vaccines; this further complicates efforts to establish a direct causal link between microchips and adverse reactions (including tumors).

Daly et al86 reported fibrosarcoma formation adjacent to an implanted microchip in a 14-year old cat. Similar to the case described above, the tumor was adjacent to, not embedded in, the tumor. Because the cat had also received numerous vaccines in the same area, the authors could not determine the origin of the fibrosarcoma. Treatment of the fibrosarcoma was multimodal, including preoperative radiation therapy and surgical resection of the mass. Histopathological evaluation of the excised mass revealed a lack of reaction around the microchip; this finding supports the likelihood that the proximity of the microchip to the tumor was coincidental, and the tumor was not induced by the presence of the implanted microchip. The report underscores the importance of following vaccination site recommendations, as described by the American Association of Feline Practitioners, to reduce the risk of sarcoma formation.

Carminato et al (2011) reported a microchip-associated fibroscarcoma in a 9-year-old cat. According to the report, the cat had never received vaccinations at the site of the tumor, supporting the author’s conclusion that the tumor was induced by the presence of the microchip. The tumor and microchip were excised, and the cat was healthy with no signs of tumor recurrence 11 months later.87

Linder et al (2009) investigated in vivo reactions in mice and in vitro reactions of feline fibroblastoid cells to implanted microchips with bioglass or polymer-coated bioglass.4 In vivo, granulomatous inflammation was observed in the tissues surrounding the microchip. The authors noted that the presence of petrolatum (added by the manufacturer for technical reasons) induced a greater granulomatous reaction than did the presence of the bioglass itself. Polypropylene caps placed on the microchips by the manufacturer to reduce migration also induced more tissue irritation. The authors concluded that “the surface material of a microchip transponder can influence the composition of the fibrous tissue capsule and the tissue reaction in vivo as well as cell growth in vitro.”5

As of 2009, in the 13 years since inception of the BSAVA's microchip adverse reaction program, 2 tumors have been reported61 despite microchip implantation in more than 3.7 million pets in the United Kingdom.61,65 The WSAVA Microchip Committee concluded that the benefits of microchip implantation far outweighed the potential health risks.17

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References

1. Saito M, Ono S, Kayanuma H et al. Evaluation of the susceptibility artifacts and tissue injury caused by implanted microchips in dogs on 1.5 T magnetic resonance imaging. J Vet Med Sci 2010; 72: 575-581.

2. Stein FJ, Geller SC, Carter JC. Evaluation of microchip migration in horses, donkeys, and mules. J Amer Vet Med Assoc 2003; 223: 1316-1319.

3. Ingwersen W. Everything you ever wanted to know about microchips. Can Vet J 1996; 37: 667-671.

4. Elcock LE, Stuart BP, Wahle BS, et al. Tumors in long-term rat studies associated with microchip animal identification devices. Exp Toxic Pathol 2001; 52: 483-491.

5.Linder M, Huther S, Reinacher M. In vivo reactions in mice and in vitro reactions in feline cells to implantable microchip transponders with different surface materials. Vet Rec 2009; 165: 45-50.

6. Ingwersen W. Microchipping of companion animals in North America. J Sm Anim Pract 2005; 46: 465.

7. Ingwersen W. Recommendations on adopting and implementing microchip technology that adheres to the ISO standards. World Small Animal Veterinary Association, 2007. Available at: http://www.wsava.org/MicrochipComm1.htm. Accessed on October 2, 2009.

8. Equine Species Working Group NAIS Recommendations to USDA, August 1, 2006. Available at: http://www.equinespeciesworkinggroup.com/info.html. Accessed September 6, 2009.

9. Hogewerf P and van ‘t Klooster K. ISO WG3 summary on the evolution of microchip technology for companion animals (November 2003). World Small Animal Veterinary Association. Available at: http://www.wsava.org/MicrochipComm2.htm. Accessed October 2, 2009.

10. Microchip identification. World Small Animal Veterinary Association Web site. Available at: http://www.wsava.org/MicrochipID.htm. Accessed October 30, 2007.

11. Lord LK, Pennell ML, Ingwerson W, et al. In vitro sensitivity of commercial scanners to microchips of various frequencies. J Amer Vet Med Assoc 2008; 233: 1723-1728.

12. Louisiana Department of Agriculture and Forestry, Equine Regulations. Available at: http://www.ldaf.state.la.us/portal/Offices/AnimalHealthServices/VeterinaryHealthDivision/EquineRegulations/tabid/347/Default.aspx. Accessed September 6, 2009.

13. Mercantel D. Micro-chipping: the wave of the future. NACA News July/August 2007: 27.

14. USDA Animal and Plant Health Inspection Service Stakeholder Report, "USDA endorses industry-recommended international standards for animal identification technologies." January 17, 2007. Available at: http://www.aphis.usda.gov/publications/animal_health/content/printable_version/sa_nais_id_technologiess.pdf. Accessed September 6, 2009.

15. Nolen RS. USDA: no authority to regulate pet microchips. Journal of the American Veterinary Medical Association. American Veterinary Medical Association Web site. Available at: www.avma.org/onlnews/javma/oct07/071015c_pf.asp. Accessed October 4, 2007.

16. USDA Animal and Plant Health Inspection Service National Animal Identification System Web site. http://animalid.aphis.usda.gov/nais/index.shtml. Accessed October 31, 2007.

17. World Small Animal Veterinary Association, WSAVA microchip survey results – Nov. 2002. Available at: http://wsava.org/MicrochipSurvey1102.htm. Accessed October 2, 2009.

18. National Companion Animal Coalition. Annex 1: Requirements to comply with the National Companion Animal Coalition (NCAC) review process regarding the use of radio frequency identification (RFID) technology in Canada. Last revised December 16, 2005. Available at: http://canadianveterinarians.net/Documents/Resources/Files/189_Annex%201_RequirementsToComply_Dec162005.pdf. Accessed October 1, 2009.

19. Department for Environment, Food, and Rural Affairs (Defra), Horse passports: new EU proposed regulation on the identification of horses. Available at http://www.defra.gov.uk/corporate/consult/horse-passports2006/letter.htm. Accessed March 22, 2007.

20. Scott-Lenon S. Dogs Trust pleased by reported support for compulsory microchipping. Dogs Trust media statement. Available at http://www.dogstrust.org.uk/press_office/pressreleases/2009/compulsory-microchipping.htm. Accessed October 1, 2009.

21. Fry R, Green R. Biological and migrational characteristics of microchips. Vet Rec 1999; 145: 564.

22. Gerber MI, Swinker AM, Staniar WB, et al. Health factors associated with microchip insertion in horses. J Eq Vet Sci 2012; 32: 177-182.

23. Platt S, Wieczorek L, Dennis R et al. Spinal cord injury resulting from incorrect microchip placement in a cat. J Fel Med Surg 2007; 9:157-160.

24. Hicks DG and Bagley RS. Imaging diagnosis – spinal injury following aberrant microchip implantation. Vet Radiol Ultrasound 2008; 49: 152-153.

25. Smith TJ and Fitzpatrick N. Surgical removal of a microchip from a puppy's spinal canal. Vet Comp Orthop Traumatol 2009; 22: 63-65.

27. Joslyn SK, Witte PG, Scott HW. Delayed spinal cord injury following microchip placement in a dog. Vet Comp Orthop Traumatol 2010; 23: 214-217.

26. van der Burgt G and Dowle M. Microchip insertion in alpacas. Vet Rec 2007; 160: 204.

28. Keeping track of microchip adverse reactions (BSAVA Report). J Small Anim Pract 2002; 43: 570.

29. Swift, S. Microchip adverse reactions. J Small Anim Pract 2000; 41: 232.

30. Lindegaard C, Vaabengaard D, Christophersen MT, et al. Evaluation of pain and inflammation associated with hot iron branding and microchip transponder injection in horses. Amer J Vet Res 2009; 70: 840-847.

31. Cohen R. Pet ID chips: a good idea? Pacific Sun Web site. Available at: http://www.pacificsun.com/news/story_print.php?story_id=234. Accessed October 2, 2009.

32. Tremayne J. Chip claim gets under oncologists' skin. Veterinary Practice News Web site. Available at: http://www.veterinarypracticenews.com/vet-cover-stories/chip-claim-gets-under-oncologists%E2%80%99-skin.aspx. Accessed October 30, 2007.

33. Lord LK, Ingwerson W, Gray JL et al. Characterization of animals with microchips entering animal shelters. J Amer Vet Med Assoc 2009; 235: 160-167.

34. Lord LK, Wittum TE, Ferketich AK, et al. Search methods that owners use to find a lost dog. J Amer Vet Med Assoc 2007; 230: 211-216.

35. Lord LK, Wittum TE, Ferketich AK, et al. Demographic trends for animal care and control agencies in Ohio from 1996 to 2004. J Amer Vet Med Assoc 2006; 229: 48-54.

36. Lord LK, Wittum TE, Ferketich AK, et al. Search methods that people use to find owners of lost pets. J Amer Vet Med Assoc 2007; 230: 217-220.

37. Lord LK, Wittum TE, Ferketich AK, et al. Search methods that owners use to find a lost cat. J Amer Vet Med Assoc 2007; 230: 1835-1840.

38. Mendoza, N. Dog home after two-year odyssey. Edwardsville Intelligencer, November 5, 2007. Available at: http://www.goedwardsville.com/site/news.cfm?newsid=18990983&BRD=2291&PAG=461&dept_id=473648&.

39. BBC News. Missing dog found after a decade. August 16, 2009. Available at: http://news.bbc.co.uk/2/hi/uk_news/england/essex/8203963.stm. Accessed September 10, 2009.

40. Stabley M and Melvin C. Virginia dog found in Oklahoma 10 years after disappearing. NBC Washington, October 1, 2009. Available at http://www.nbcwashington.com/news/local-beat/Virginia-Dog-Found-in-Oklahoma-10-Years-After-Disappearing-62957122.html. Accessed October 1, 2009.

41. Stein L. The incredible journey: microchip ID reunites owners with cat – 13 years later. Scientific American, November 14, 2008. Available at: http://www.scientificamerican.com/article.cfm?id=the-incredible-journey-microchip-id-reunites-cat-with-owners. Accessed October 2, 2009.

42. Montagne R. Dallas woman's dog turns up in Florida. NPR, August 20, 2009. Available at: http://www.npr.org/templates/story/story.php?storyId=112052510. Accessed September 10, 2009.

43. myFOXphoenix.com. Dog reunited with owner after 600-mile journey. August 31, 2009. Available at: http://www.myfoxphoenix.com/dpp/news/pets/calif_dog_reunited_08_31_2009. Accessed September 10, 2009.

44. Dale S. Vanessa Williams, pet lover. TwinCities.com-Pioneer Press. Available at: http://www.freep.com/apps/pbcs.dll/article?AID=/20071115/FEATURES10/71115076/1188. Accessed November 18, 2007.

45. Hudson LC, Berschneider HM, Ferris KK, et al. Disaster relief management of companion animals affected by the floods of Hurricane Floyd. J Amer Vet Med Assoc 2001; 218: 354-359.

46. Beaver BV, Gros R, Bailey EM, et al. Report of the 2006 National Animal Disaster Summit. J Amer Vet Med Assoc 2006: 229: 943-948.

47. McConnico RS, French DD, Clark B, et al. Equine rescue and response activities in Louisiana in the aftermath of Hurricanes Katrina and Rita. J Amer Vet Med Assoc 2007; 231: 384-392.

48. Greenwood P. Animal identification and the veterinary practitioner. Can Vet J 2004; 45: 332-333.

49. Robinson TR, Hussey SB, Hill AE, et al. Comparison of temperature readings from a percutaneous thermal sensing microchip with temperature readings from a digital rectal thermometer in equids. J Amer Vet Med Assoc 2008; 233: 613-617.

50. USDA Animal and Plant Health Inspection Service, report to The Honorable Robert C. Byrd, July 2007. Available at: http://www.amacausa.org/UserFiles/File/USDA%20Microchip%20Report.pdf. Accessed October 2, 2009.

51. Nolen RS. Pet's death rekindles electronic ID debate. Journal of the American Veterinary Medical Association, 2004. American Veterinary Medical Association Web site. Available at: http://www.avma.org/onlnews/javma/jul04/040701a.asp. Accessed October 30, 2007.

52. World Small Animal Veterinary Association, United States microchip report – 2006. Available at: http://www.wsava.org/MicrochipID.htm. Accessed October 2, 2009.

53. Coalition for Reuniting Pets and Families, Readallchips.org. Available at: http://www.avma.org/readallchips/default.asp. Accessed October 30, 2009.

54. Bayer HealthCare Animal Health Division press release. Available at: http://www.resq.petparents.com/bayerCommitment.cfm. Accessed November 27, 2007.

55. Kahler S. Tags on their collar, chips in their shoulder. 144th AVMA Ann Conv Daily News; Wednesday, July 18, 2007. Available at: http://www.avma.org/convention/news/wednesday17.asp. Accessed November 27, 2007.

56. Lord LK, Pennell ML, Ingwerson W, et al. Sensitivity of commercial scanners to microchips of various frequencies implanted in dogs and cats. J Amer Vet Med Assoc 2008; 233: 1729-1735.

57. Lord LK, Griffin B, Slater MR et al. Evaluation of collars and microchips for visual and permanent identification of pet cats. J Amer Vet Med Assoc 2010; 237: 387-394.

58. Boutin H. It's raining cats and dogs and RFID tags. RFID Product News, 2007. Available at: www.rfidproductnews.com/issues/2007.09/raincatsdogs.php. Accessed November 8, 2007.

59. Merrihew J. Unprecedented Collaboration Helps Lost Pets Reunite with Owners: AAHA unveils new microchip look-up tool. American Animal Hospital Association press release, September 21, 2009. Available at: https://secure.aahanet.org/eweb/dynamicpage.aspx?site=media&webcode=prdetail&postKey=ce02e804-8b88-4e3f-87c8-fd5fc8fa9182. Accessed October 2, 2009.

61. Personal communication, British Small Animal Veterinary Association, September 10, 2009.

62. Collating data on adverse reactions to microchips. J Small Anim Pract 2004; 45: 644-645.

63. British Small Animal Veterinary Association Web site. Available at: http://www.bsava.com/resources/microchipadvice/. Accessed November 2, 2007.

64. Jansen JA, van der Waerden JPCM, Gwalter RH, et al. Biological and migrational characteristics of transponders implanted into beagle dogs. Vet Rec 1999; 145: 329-333.

65. Petlog. Available at: http://www.thekennelclub.org.uk/petlog/. Accessed October 2, 2009. Available at: http://www.amacausa.org/UserFiles/File/USDA%20Microchip%20Report.pdf. Accessed October 2, 2009.

66. Lambooij E, de Groot PHS, Molenbeek RF, et al. Subcutaneous tissue reaction to polyethylene terephtalate-covered electronic identification transponders in pigs. Vet Q 1992; 14: 145-147.

67. Gruys E, Schakenraad JM, Kruit LK, et al. Biocompatibility of glass-encapsulated electronic chips (transponders) used for the identification of pigs. Vet Rec 1993; 133: 385-388.

68. Conill C, Caja G, Nehring R, et al. Effects of injection position and transponder size on the performances of passive injectable transponders used for the electronic identification of cattle. J Anim Sci 2000; 78: 3001-3009.

69. Haifley KA, Hecht S. Functionality of implanted microchips following magnetic resonance imaging. J Amer Vet Med Assn 2012; 240: 577-579.

70. Brand KG, Johnson KH, Buoen LC, et al. Foreign body tumorigenesis. CRC Critical Rev Toxicol 1976; 4: 353-394.

71. Tillmann T, Kamino K, Dasenbrock C, et al. Subcutaneous soft tissue tumours at the site of implanted microchips in mice. Exp Toxic Pathol 1997; 49: 197-200.

72. Palmer TE, Nold J, Palazzolo M, et al. Fibrosarcomas associated with passive integrated transponder implants. [abstract] Toxicol Pathol 1998; 26: 170.

73. Blanchard KT, Barthel C, French JE, et al. Transponder-induced sarcoma in the heterozygous p53+/- mouse. Toxicol Pathol 1999; 27: 519-527.

74. Johnson KH, Ghobrial HKG, Buoen LC, et al. Nonfibroblastic origin of foreign body sarcomas implicated by histological and electron microscope studies. Cancer Res 1973; 33: 3139-3154.

75. Johnson KA. Foreign-body tumorigenesis: sarcomas induced in mice by subcutaneously implanted transponders. [abstract] Vet Pathol 1996; 33: 619.

76. Vascellari M, Melchiotti E, Mutinelli F. Fibrosarcoma with typical features of postinjection sarcoma at site of microchip implant in a dog: histologic and immunohistochemical study. Vet Pathol 2006; 43: 545-548.

77. Vascellari M, Mutinelli F, Cossettini R, et al. Liposarcoma at the site of an implanted microchip in a dog. Vet J 2004; 168: 188-190.

78. Ball DJ, Argentieri G, Krause R, et al. Evaluation of a microchip implant system used for animal identification in rats. Lab Anim Sci 1991; 41: 185-186.

79. Siegal-Willott J, Heard D, Sliess N, et al. Microchip-associated leiomyosarcoma in an Egyptian fruit bat (Rousettus aegyptiacus). J Zoo Wildlife Med 2007; 38: 352-356.

80. Rao GN, Edmondson J. Tissue reaction to an implantable identification device in mice. Toxicol Pathol 1990; 18: 412-416.

81. Le Calvez S, Perron-Lepage M-F, Burnett R. Subcutaneous microchip-associated tumours in B6C3F1 mice: a retrospective study to attempt to determine their histogenesis. Exper Toxic Pathol 2006; 57: 255-265.

82. Pessier AP, Stalis IH, Sutherland-Smith M, et al. Soft tissue sarcomas associated with identification microchip implants in two small zoo animals. Proc Amer Assoc Zoo Vet 1999: 139-140.

83. Murasugi E, Koie H, Okano M, et al. Histological reactions to microchip implants in dogs. Vet Rec 2003; 153: 328-330.

84. Vaccine-Associated Feline Sarcoma Task Force. The current understanding and management of vaccine-associated sarcomas in cats. J Amer Vet Med Assoc 2005; 226: 1821-1842.

85. McCarthy PE, Hedlund CS, Veazy RS et al. Liposarcoma associated with a glass foreign body in a dog. J Amer Vet Med Assoc 1996; 209: 612-614.

86. Daly MK, Saba CF, Crochik SS et al. Fibrosarcoma adjacent to the site of microchip implantation in a cat. J Feline Med Surg 2008; 10:202-5.

87. Carminato A, Vascellari M, Marchioro W et al. Microchip-associated fibrosarcoma in a cat. Vet Derm 2011; 22: 565-569.

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